Banca de QUALIFICAÇÃO: ANDRESSA FRANCINE MARTINS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANDRESSA FRANCINE MARTINS
DATE: 06/03/2024
TIME: 14:00
LOCAL: Campus de São Bernardo do Campo - remoto
TITLE:

Biological characterization of titanium alloys used in medical applications

PAGES: 76
BIG AREA: Engenharias
AREA: Engenharia Biomédica
SUBÁREA: Engenharia Médica
SPECIALTY: Biomateriais e Materiais Biocompatíveis
SUMMARY:

Bone tissue is a type of specialized connective tissue contained by cells and a calcified extracellular matrix. It has intrinsic regenerative capacity, high stiffness and low flexibility, characteristic of human bones and which helps support soft tissues, protect vital organs and support the skeleton. In the case of injury or pathology, replacement by scar tissue and subsequent bone remodeling may occur, filling the area with newly formed tissue. However, in specific cases or with the involvement of large areas, it may be necessary to use biomaterials that promote bone regeneration and that also maintain the functional structure, associated or not with metallic structures, such as plates and pins, to stabilize bone tissue. affected bone ends. Therefore, this work seeks to study the biological interaction of Ti CP 2 for application in bone tissues. Ti CP2 samples were used for the cell interaction test, from Vero lineage cells, maintained in culture with HAMF medium, 10% fetal bovine serum and antibiotics. A total of 93.9% cell viability was achieved. 10,000 cells were inoculated into culture plates on Ti CP2 samples. The cells were maintained in culture for 24 hours to evaluate the initial cellular interaction of the cells with the titanium samples. Positive (latex) and negative (ideal culture conditions) controls were used. After this period, cells were observed in contact with Ti CP2, using phase contrast light microscopy to characterize the cytotoxicity of the samples. In the qualitative analysis of cytotoxicity, inverted light microscopy with phase contrast was used, in order to identify, in general, changes in morphology or also the incidence of cell lysis or even cytoplasmic vacuolization. The results obtained show potential for the use of Ti CP2 as an alternative in biomedical applications, as a good cellular response was observed, without signs of cytotoxicity, demonstrating them as biologically compatible.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 1760474 - PATRICIA APARECIDA DA ANA
Membro Titular - Examinador(a) Externo ao Programa - 3327250 - MÔNICA HELENA MONTEIRO DO NASCIMENTO
Membro Titular - Examinador(a) Externo ao Programa - 2297395 - FREDERICO AUGUSTO PIRES FERNANDES
Membro Suplente - Examinador(a) Interno ao Programa - 2090031 - ILKA TIEMY KATO PRATES
Membro Suplente - Examinador(a) Externo ao Programa - 1718113 - ANA PAULA ROMANI
Membro Suplente - Examinador(a) Externo à Instituição - FELIPE NOGUEIRA AMBROSIO