Development of gelatin-based hydrogel associated with tricalcium phosphate and methylene blue for use in bone grafting
The search for biomaterials and techniques that minimize the number of surgical interventions and that provide an efficient repair is still necessary. In this sense, biomaterials that promote the repair of hard and soft tissues, and that associate the activity of simultaneous decontamination of the surgical site become interesting mainly in intraoral bone grafting procedures, considering that the oral cavity has microorganisms that can compromise the success of the procedure . Thus, this study aimed to develop a biomaterial that facilitates a bone grafting procedure with simultaneous local decontamination. The proposed biomaterial has a gelatin base associated with tricalcium phosphate (TCP), a potent agent that promotes bone remineralization, and methylene blue, a photosensitizer used in antimicrobial photodynamic therapy (aPDT). For this, dense films of gelatin with TCP were prepared using the technique of dissolving gelatin in 2,2,2 trifluoroethanol-5% TFE solvent. Afterwards, the incorporation of 0.005% methylene blue was performed by means of swelling. The characterization of the films was performed by scanning electron microscopy (SEM), swelling tests, Fourier transform infrared absorption spectroscopy (FTIR) and thermogravimetric analysis (TGA). Cytotoxicity tests were also carried out using Vero cells, as well as microbial reduction tests with S. aureus. It was possible to notice the formation of a stable film, with a predominance of TCP in the lower portion, and uniform blue coloration, which suggests the incorporation of both solutes in the gelatin membrane. The proof of this incorporation was confirmed by FTIR and SEM analyses. Such films were not cytotoxic in the absence of light; however, after irradiating the films with a red laser at a wavelength of 660 nm, more than 90% of bacterial reduction was observed. It can be concluded that the developed hydrogel is promising for future clinical application.