TOXICOLOGY EVALUATION OF BIOINK IN SPHEROID MODEL
The development of bioinks for printing cells in three-dimensional models has been the subject of study for the new technological generation. The bioinks need to interact with the cells to promote their viability for as long as possible, which currently has been a bottleneck in the application of this technique. The development of a biotint must take into account the physical and chemical properties of the components and their interaction with living cells, characteristics that are sometimes not achieved. To understand the relationship between cells and bioinks compounds, this work aims to evaluate the viability and functionality of cells cultured in spheroids. Spheroids were prepared using NIH-3T3 cells (mouse fibroblasts) in micro molds. They were characterized as to their diameter and identification of the cells that compose them to develop an analysis by convolutional neural network. It was possible to obtain different sizes of spheroids and start training the convulational neural network. These characterization studies will be applied in the future to evaluate these spheroids when grown with bioinks.