Banca de QUALIFICAÇÃO: LEONARDO DE SOUZA
Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.DISCENTE : LEONARDO DE SOUZA
DATA : 16/08/2019
HORA: 10:00
LOCAL: Na sala 301, 3º andar, Bloco B, Campus SA da Fundação Universidade Federal do ABC, localizada na Avenida dos Estados, 5001, Santa Terezinha, Santo André, SP
TÍTULO:
Desenvolvimento e Caracterização de Arcabouços fibrosos a base de Blendas Poliméricas de Pululana e PVA, por rotofiação, para Aplicações em Engenharia de Tecido de Pele
PÁGINAS: 60
RESUMO:
The main hindrances for cost effective skin injury treatments are tightly associated to the limited number of donors and the immunogenic reactions triggered by antigens present in the grafts. Beside the intense research and development in tissue engineering and regenerative medicine, Human Skin Equivalents (HSE) have been proposed both as clinical skin substitutes, due to their biocompatibility and non-immunogenicity, and models for permeation and toxicity studies. Since the last decades, the investigations of scaffolds, as framework environments for cell growth, have shown a boom of interest. It seems undoubtedly that a fibrous three-dimensional structure may reliably mimic the extracellular matrix. A large number of techniques has been used to produce fibrous mats, among which Rotary Jet-Spinning (RJS) appears undoubtedly as one of the most efficient. However, an important open challenge remains for the optimization of experimental design to get a fine control of the fibers morphology and size, as well as for the mat homogeneity and reproducibility. In this work, we present an experimental study on the formation process of polymer fibrous scaffolds, based on a biopolymer, Pullulan - PULL, and a synthetic polymer, Poly (vinyl alcohol) - PVA, by means of Rotary Jet-Spinning (RJS) technique. We have analyzed the influence of the polymer composition (X1), solvent volatility (X2) and RJS collector distance (X3) on the fibers diameter (Y). The ranges of independent variables were 0:100–0:100 for the PULL:PVA blend composition, 0–100% for DMSO percentage in DMSO/H2O solvent mixture, 4–9 cm for collector distance. To minimize the number of required experiments for a complete evaluation, Response Surface Methodology (RSM) and Central Composite Rotatable Design (CCRD) have been performed using the Expert Design Software. The nanofiber morphology and thermal properties have been analyzed by scanning electron microscopy (SEM) and thermogravimetric measurements, respectively. It was found that the fibers diameter ranged from 300 nm to 1500 nm, depending on the for collector distance, and tightly on the rheological properties of polymer solution. The RSM analysis showed that a quartic model could best fit the experimental results.
MEMBROS DA BANCA:
Presidente - Interno ao Programa - 1552434 - LUCIANO AVALLONE BUENO
Membro Titular - Examinador(a) Interno ao Programa - 1544341 - WENDEL ANDRADE ALVES
Membro Titular - Examinador(a) Externo ao Programa - 1675708 - DANIELE RIBEIRO DE ARAUJO
Membro Suplente - Examinador(a) Interno ao Programa - 1600878 - ARNALDO RODRIGUES DOS SANTOS JUNIOR
Membro Suplente - Examinador(a) Externo ao Programa - 1676364 - JULIANA MARCHI