Banca de DEFESA: ANDRE LUIZ MARQUES DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANDRE LUIZ MARQUES DE SOUZA
DATA : 17/09/2019
HORA: 09:00
LOCAL: Auditório, 8º andar, Bloco B, Campus SA da Fundação Universidade Federal do ABC, localizada na Avenida dos Estados, 5001, Santa Terezinha, Santo André, SP
TÍTULO:

Development of Single Particle ICP-MS Method for Silver Nanoparticles sizing on Biological Matrices

PÁGINAS: 50
RESUMO:

Nanotechnology has presented a huge grew on last decades as component of several products and applications on the contemporaneous society, and the nanomaterials can be defined as a suspension where 50% from the materials are found between 10-100 nm on at least 1 dimension. Within the nanomaterials types, can be described the Carbon-based nanomaterials such as carbon nanotubes and the Graphene, the Organic based nanomaterials such as Liposomes and proteic nanomaterials and the Inorganic based nanomaterials, such as the Quantum-dots and the metallic nanoparticles, where the Silver Nanoparticles, AgNPs, are found. Its application go by pesticides, food addictive, make up and skincare products and electronic compounds for instance. Although their presence on market is consolidate, the Toxicological and environmental effects of are unknown, and for this studies and control, its monitoring necessary, but however, traditional analytical techniques for metallic nanoparticles, such as Dynamic Light Scattering, Atomic Force Microscopy and Nanoparticle Tracking Analysis presents some disadvantages as matrices interferences, low limits of detection and low analytical frequency; on this situation the Inductively Coupled Plasma Mass Spectrometer, ICP-MS, an analytical technique widely used for metals detection and quantification on several matrices and samples, presenting the lowest detection limits within metal analytical techniques, presented itself for a complement as a new tool for metallic nanoparticles detection, sizing and quantification, by its operation though the Single Particle mode, where it is considered on every detection event, a unique nanoparticle aggregate is detected, and this event is related to the NP size and concentration. On the present work, it was studied the influence of human matrices: blood plasma, sweat, urine and saliva, on detection and sizing of commercial AgNPs. First, it was observed a 20-fold matrice dilution, the same commonly used for urine and plasma clinical analysis, was able to allow the detection of AgNPs and its sizing. On second place, it was observed the behavior of the AgNPs on the matrice on different concentrations: from 0.1 to 2 mg L^-1, the material presented itself a  small degradation and a good detection, and from 5 to 10 mg L^-1 an expansive degradation and consequently a bad detection; it was also observed the AgNPs presented different behaviours on the matrices, due its composition: blood plasma and saliva aggregated it and sweat made it on smaller sizes.  On third place, it was observed the  urine and plasma preparations could be storage on -5 C up to 7 days, for further analysis, been compatible with clinical tests procedures, where the samples almost never can be analysed at the same collect event.

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 2046361 - BRUNO LEMOS BATISTA
Membro Titular - Examinador(a) Externo ao Programa - 2999257 - NELSON EDUARDO DURAN CABALLERO
Membro Titular - Examinador(a) Externo à Instituição - MÁRCIA ANDREIA MESQUITA SILVA DA VEIGA
Membro Suplente - Examinador(a) Externo ao Programa - 1933328 - HELOISA FRANCA MALTEZ
Membro Suplente - Examinador(a) Externo à Instituição - MÔNICA HELENA MONTEIRO DO NASCIMENTO