Enhancement of anaerobic digestion by conductive materials via direct interspecies electron transfer: a study of carbonaceous materials
Anaerobic Digestion (AD) is a fundamental technology for energy and resource recovery within the circular bioeconomy. However, its operational efficiency is frequently constrained by the slow conversion kinetics of intermediates, such as Volatile Fatty Acids (VFAs), which constitutes a metabolic bottleneck. This thesis investigates the application of conductive materials as a strategy to intensify the AD process by promoting Direct Interspecies Electron Transfer (DIET), a more thermodynamically favorable pathway. The research is structured around two complementary approaches. Firstly, a long-term performance evaluation of Carbon Felt (CF) as a conductive support medium in an Anaerobic Membrane Bioreactor (AnMBR). The CF-supplemented reactor demonstrated a significant acceleration in the start-up phase, consistent with DIET stimulation, but did not sustain production superiority in the long term, suggesting that the material's biocompatibility might override conductivity as the limiting factor. Secondly, the investigation delves into the mechanism of action through a matrix of carbonaceous materials (Hydrochar (HC), Biochar (BC), and their iron-doped versions (HC-Fe, BC-Fe)). This study aims to correlate key physicochemical properties (electrical conductivity vs. redox-active surface sites) with methanogenic performance in Biochemical Methane Potential (BMP) assays using propionate as a model substrate. The goal is to understand whether AD enhancement is primarily mediated by electron conduction through the carbon structure (BC) or by redox mediation via oxygen-containing functional groups (HC and Fe-doped materials), which will guide the future development of sustainable carbon additives for real-world wastewater treatment.