Banca de QUALIFICAÇÃO: VICTOR AUGUSTO GENEROSO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : VICTOR AUGUSTO GENEROSO
Data: 03/08/2026
HORA: 14:00
LOCAL: remoto
TÍTULO:

 

THERAPEUTIC EFFECTS OF PHOTOBIOMODULATION ON HIPPOCAMPAL NEUROGENESIS IN A MODEL OF EARLY NEURODEGENERATION FOLLOWING NEONATAL ASPHYXIA

PÁGINAS: 50
RESUMO:

Perinatal asphyxia (or neonatal asphyxia) is a major cause of perinatal brain injury and is associated with persistent cognitive and neurobiological impairments. Photobiomodulation (PBMT) has emerged as a promising therapeutic strategy because of its effects on mitochondrial metabolism, oxidative stress, and neuroinflammation. This study aimed to establish a neonatal anoxia model in SAMP8 mice and to investigate the therapeutic effects of PBMT on behavioral and histological outcomes. Three-day-old male SAMP8 mice were subjected to neonatal anoxia by exposure to 100% nitrogen for different durations to standardize the model. Injury severity was assessed using an adapted APGAR score and Fluoro-Jade C staining for neuronal degeneration. The effects of PBMT were evaluated by Iba1 immunofluorescence and behavioral assessment in the Morris Water Maze. The 12-min protocol produced consistent acute neurological impairment, characterized by reduced APGAR scores and increased neuronal degeneration, and was therefore selected for the subsequent experiments. Neonatal anoxia predominantly impaired the acquisition phase of spatial learning, whereas long-term spatial memory retention was largely preserved. PBMT attenuated this delay in learning acquisition, resulting in behavioral performance that more closely resembled that of control animals during the initial training sessions. Although no significant differences were detected within most individual hippocampal subregions, PBMT reduced the overall number of Iba1-positive cells throughout the hippocampus. Collectively, these findings establish a reproducible neonatal anoxia model in SAMP8 mice and provide preliminary evidence that PBMT attenuates behavioral deficits while modulating the hippocampal microglial response following neonatal brain injury. Additional analyses of neurogenesis, neuronal morphology, oxidative stress, and astroglial activation are currently underway and are expected to further clarify the mechanisms underlying these effects.

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 1675707 - FULVIO RIELI MENDES
Membro Titular - Examinador(a) Externo à Instituição - FELIPE JOSÉ COSTA VIANA
Membro Titular - Examinador(a) Externo à Instituição - GLAUCIA MONTEIRO DE CASTRO - UNIFESP
Membro Suplente - Examinador(a) Interno ao Programa - 1676367 - ALEXANDRE HIROAKI KIHARA