Wastewater-based epidemiology as a strategy for community monitoring, mapping emerging foci, and designing early warning systems for SARS-CoV-2
SARS-CoV-2, the new Coronavirus, was first detected in Wuhan, China, in December 2019, and has since spread rapidly, causing millions of deaths worldwide. Several studies have reported faecal clearance of SARS-CoV-2 RNA titers from symptomatic and asymptomatic infected individuals. Therefore, quantification of SARS-CoV-2 in wastewater can be used to track the spread of the virus in a population. In this study, we present the methodology developed for the concentration of sewage samples, extraction and detection of viral RNA in real-time PCR and the results of concentration and viral load found during monitoring carried out for more than 01 (one) year in five points of sampling in the ABC region (São Paulo, Brazil).