Banca de QUALIFICAÇÃO: JOSE MIGUEL ZUÑIGA PRADO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : JOSE MIGUEL ZUÑIGA PRADO
DATA : 19/10/2021
HORA: 14:00
LOCAL: https://meet.google.com/iez-xvei-maq
TÍTULO:

SYNTHESIS AND CHARACTERIZATION OF RGO FILMS FOR
MANUFACTURING OF ELECTRODES AND THEIR APPLICATION IN
BIOSENSORS

PÁGINAS: 68
GRANDE ÁREA: Engenharias
ÁREA: Engenharia de Materiais e Metalúrgica
RESUMO:

The development of biosensors focusing on the use of rGO (reduced graphene oxide) in the manufacture of electrodes for virus detection is due to the multiple properties that this material has and that it has better performance than graphene oxide (GO) and graphene, in the sense of its practicality for its use in the interaction with biological material and its ease of synthesis, which is demonstrated in this research. Several investigations have addressed the use of rGO in the development of transducer systems, but with low repeatability and scalability. Objective: to manufacture a transducer system that is functional, that is, that can, in the first instance, record biological events and, in the second instance, that is capable of differentiating these events; that is affordable and easily industrialized. To achieve this goal, this research also helps to understand the characteristics of the electrode developed for its performance without the transduction process during its application as an electrochemical sensor. Methods: A methodology begins with the development of techniques for the production of thin films, dividing this step mainly into metallic (copper and gold) and non-metallic (cysteine and Ly-rGO) thin films. To be sure to continue on the right path, we chose to carry out characterization tests, both of the raw material (graphite) and of the films produced, tests that also helped us to evolve and confirm the electrode designs and their detection capacity. Furthermore, in the development of the research, team meetings and experimentation were continuously used as a common practice for the development of techniques. We obtained rGO in a stable and good quality solution with Lysozyme and Hidrazine, we could easily differentiate graphite from GO and rGO, we obtained a multifunctional and multilayer transducer system, we achieved a micro-fluidic system solidly integrated with the transducer system, we were able to manufacture an immersion coating equipment. The main conclusions are that the rGO obtained, associated with Lysozyme and it can be used on an industrial scale for the deposition of films on a Cu/Au/Cy (Cysteine) system, due to its high stability in aqueous solution. It can be concluded, after cyclic voltammetry processes, that the electrode is stable and through chronoamperometry that the electrode helps in the transduction process, which is sought for the identification of viruses in the biosensor

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 2604737 - EVERALDO CARLOS VENANCIO
Membro Titular - Examinador(a) Interno ao Programa - 1676364 - JULIANA MARCHI
Membro Titular - Examinador(a) Externo ao Programa - 1844792 - AMEDEA BAROZZI SEABRA
Membro Suplente - Examinador(a) Interno ao Programa - 1646041 - CARLOS TRIVENO RIOS
Membro Suplente - Examinador(a) Interno ao Programa - 1761120 - DANILO JUSTINO CARASTAN