Banca de QUALIFICAÇÃO: JULIANA NAOMI YAMAUTI COSTA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JULIANA NAOMI YAMAUTI COSTA
DATE: 14/12/2023
TIME: 14:00
LOCAL: Remoto
TITLE:

Ultra-dense vertical electrochemical biosensors enable single-response multiplexed diagnosis

PAGES: 90
BIG AREA: Ciências Exatas e da Terra
AREA: Química
SUBÁREA: Química Analítica
SPECIALTY: Eletroanalítica
SUMMARY:

The central problem motivating this project is the rapid, accessible, and accurate diagnosis of diseases, either in a laboratory setting or at the point of need. In this context, platforms with high testing capacity emerge as essential tools to expedite diagnosis and enhance clinical accuracy, enabling the simultaneous and sequential assessment of distinct biomarkers. Electrochemical label-free biosensors (BELF) offer high analytical performance combined with speed, simplicity, portability, and low cost, but they have not yet successfully reached the commercial market beyond the personal blood glucose assays. In response to this gap, we present a new configuration of an electrochemical device that (i) performs serial analysis of a high number of sensors, (ii) combines high reproducibility with simplicity of use and reduced cost, and (iii) introduces something unprecedented in electrochemistry: the simultaneous and independent monitoring of distinct redox reactions from a single square wave voltammetry (SWV) scan, using a single working electrode (WE). Abbreviated as SERM (single-response multiplexing), this strategy promises to propel advances in multiplexed systems by enabling the use of BELFs for the simultaneous detection of multiple analytes from single-channel portable potentiostats. Produced through microfabrication techniques, the sensors consist of only 2 electrodes comprising vertical thin films of Au separated by a dielectric (SU-8) and arranged in a mesh. This configuration contributes to the integration capacity of sensors (many sensors per area), resulting in a significant reduction in the final cost of chips. Considering an industrial infrastructure and 48 sensors per wafer, the unit cost was estimated at R$3.55. In this study, as a proof of concept, we evaluated the device's performance as a biosensor by modifying the WEs with peptides (recognition element) for the detection of COVID-19 IgG antibodies in real samples. Through this innovative approach, we were able to double processing capacity while simultaneously achieving 100% accuracy in serum samples. We envision the platform to enable broad applications in high-testing capacity approaches, as it can be tailored to other biosensing devices and formats.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 1544341 - WENDEL ANDRADE ALVES
Membro Titular - Examinador(a) Externo à Instituição - BRUNO CAMPOS JANEGITZ - UFSCAR
Membro Titular - Examinador(a) Externo à Instituição - LAÍS CANNIATTI BRAZACA - USP
Membro Suplente - Examinador(a) Interno ao Programa - 050.966.059-22 - MURILO SANTHIAGO - CNPEM
Membro Suplente - Examinador(a) Externo à Instituição - WILLIAM REIS DE ARAUJO - UNICAMP
Membro Suplente - Examinador(a) Externo à Instituição - PAULO ROBERTO BUENO - UNESP