Banca de DEFESA: VANESSA FUENTES SUGUIYAMA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : VANESSA FUENTES SUGUIYAMA
DATA : 13/12/2019
HORA: 14:00
LOCAL: sala S17, Térreo, Bloco Delta, Campus SBC da Fundação Universidade Federal do ABC, localizada na Alameda da Universidade, s/n, Bairro Anchieta - São Bernardo do Campo, SP
TÍTULO:

Understanding the responses to the water stress in Setaria italica and the influence of the transposable elements

PÁGINAS: 281
GRANDE ÁREA: Ciências Biológicas
ÁREA: Genética
SUBÁREA: Genética Vegetal
RESUMO:

The development of plants in resource-constrained conditions, such as water stress, is possible through mechanisms that confer phenotypic plasticity. Thus, under adverse conditions, to a greater or lesser extent, all plants respond by altering metabolism, which leads to changes in vegetative and reproductive traits. The plant strategies to tolerate water stress are often associated with modifications in gene expression patterns, which can be given by both genetic and epigenetic changes. Transposable elements (TEs) are repetitive DNA sequences able to insert in new positions of the genome, generating mutations and altering epigenetic patterns, thus playing an important role in the evolution of the species. However, the relationships between the impact of TE mobilization on the genome and its contribution to phenotypic plasticity, whether of genetic or epigenetic origin, are not completely elucidated. In this context, the present sutidy proposed to determine the mechanisms related to phenotypic plasticity in the Setaria italica (Poaceae) model species in response to water stress. Therefore, we sought to evaluate the plastic responses at different stress levels through phenotypic, physiological and gene expression (mRNAs and sRNAs transcriptomes) analysis, as well as to associate differential gene expression with epigenetic alterations promoted by the mobilization of TEs. To meet these demands, the first chapter evaluated the diversity and evolution of LTR retrotransposons by analyzing the elements of the S. italica sequenced genome. Additionally, seeking a better understanding of the evolutionary history in the different LTR-RT lineages, we propose a new approach to the study of the elements individually. To evaluate the phenotypic plasticity at different stress intensities, in the second chapter, plants were cultivated under different water regimes, in which vegetative and reproductive traits and gene expression profile of mRNAs and sRNAs were evaluated. Finally, to validate the hypothesis of the influence of epigenetic mechanisms modulated by the presence of TEs in response to water stress, we correlated the presence of TEs with differences in gene expression patterns by comparing sRNA profiles in genes and their putative regulatory regions. These results will allow us to broaden the knowledge about evolution of adaptation to environmental stress and the impact of TEs in this process.

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 1912347 - NATHALIA DE SETTA COSTA
Membro Titular - Examinador(a) Externo ao Programa - 1948411 - LUIZ ROBERTO NUNES
Membro Titular - Examinador(a) Externo à Instituição - MARIE-ANNE VAN SLUYS - USP
Membro Titular - Examinador(a) Externo à Instituição - LUCIANO FRESCHI - USP
Membro Titular - Examinador(a) Externo à Instituição - DOUGLAS SILVA DOMINGUES - UNESP
Membro Suplente - Examinador(a) Externo ao Programa - 3065803 - WAGNER RODRIGO DE SOUZA
Membro Suplente - Examinador(a) Externo à Instituição - MARCELO MARQUES ZERILLO - USP