Banca de DEFESA: IÊDA CAROLINA MANTOVANI CLARO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : IÊDA CAROLINA MANTOVANI CLARO
DATE: 31/05/2023
TIME: 14:00
LOCAL: remoto
TITLE:

Wastewater-based epidemiology as a strategy for community monitoring, mapping emerging foci, and designing early warning systems for SARS-CoV-2

PAGES: 50
BIG AREA: Engenharias
AREA: Engenharia Sanitária
SUBÁREA: Saneamento Ambiental
SPECIALTY: Microbiologia Aplicada e Engenharia Sanitária
SUMMARY:

The coronavirus pandemic has caused deaths around the world, mainly due to its easy spread through contact and because it is a respiratory virus. Studies indicate the presence of virus RNA in wastewater collection systems, considering that a contaminated individual eliminates the virus through feces and urine. Monitoring and quantification of the SARS-CoV-2 virus in wastewater makes it possible to also count asymptomatic cases of the disease and assists in the public management of the pandemic. Thus, the objective of this research is to carry out the epidemiological mapping of the SARS-CoV-2 virus in the wastewater system of specific regions of Greater ABC - SP. The collected samples were concentrated through precipitation with PEG 8000 and centrifugation, the RNA was extracted with an extraction kit and virus detection and quantification was performed through real-time RT-qPCR. The results were used to generate a spatial and temporal georeferenced mapping of the spread of the virus in order to help monitor the behavior of the virus in the region. It was also possible to verify a pattern of anticipation of the behavior of clinical cases in up to 2 weeks. The methodology proved to be capable of assisting in the epidemiological monitoring of the region, but there are still some aspects to be explored, such as the genetic sequencing of the virus and the existence of a correlation between the viral concentration detected in wastewater and the physicochemical characteristics of the effluent.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 2342998 - RODRIGO DE FREITAS BUENO
Membro Titular - Examinador(a) Interno ao Programa - 1646410 - ROSELI FREDERIGI BENASSI
Membro Titular - Examinador(a) Externo à Instituição - CAROLINE RIGOTTO
Membro Suplente - Examinador(a) Interno ao Programa - 1814655 - LUCIA HELENA GOMES COELHO
Membro Suplente - Examinador(a) Externo ao Programa - 1222415 - MERCIA REGINA DOMINGUES MORETTO