Banca de DEFESA: FERNANDA MARIA RODRIGUEZ

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : FERNANDA MARIA RODRIGUEZ
DATA : 06/03/2020
HORA: 14:00
LOCAL: sala 307, 3º andar, Bloco B, Campus SA da Fundação Universidade Federal do ABC, localizada na Avenida dos Estados, 5001, Santa Terezinha, Santo André, SP
TÍTULO:

Development of Methodology and Accessories for the Production of Restricted Access Material (RAM), using the Solid Phase Peptide Synthesis Technique (SPPS): Application in HPLC and Evaluation of the Enantioselectivity of Material.

PÁGINAS: 212
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Química de Macromoléculas
ESPECIALIDADE: Proteínas
RESUMO:

A new restricted access material (RAM) is presented in this work. A peptide with L and D-amino acids was designed and synthesized to give the material attractive chiral sites for analytes capable of forming enantioseparation-friendly intermediate complexes. The developed peptide was cross-linked with glutaraldehyde and supported on ODS-C18 and used in reverse phase HPLC column. Thus, a material was developed that allowed not only the exclusion of macromolecules, but also the maintenance of the excellent partition characteristics found in reverse phase materials. There is also an innovation not presented in the literature, namely the resolution of complex matrix analytes through direct injection of paraben-reinforced bovine serum, showing the maintenance of reverse phase characteristics, retention and separation with optimal resolution of the analytes tested. With the same column it was also possible to separate a chiral pattern of 5-methyl-5-phenylhydantoin, demonstrating that it was possible to design a peptide with specific characteristics, allowing greater affinity of the RAM phase with the analyte of interest. Therefore, the developed material brings a new tendency to the restricted access materials, being possible to choose residues that favor material specific characteristics, bringing a new status to the RAM columns, since it allowed the direct injection of complex samples, and separation of the analytes in different arrays in a single dimension, that is, in a single column.

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 1544344 - VANI XAVIER DE OLIVEIRA JUNIOR
Membro Titular - Examinador(a) Interno ao Programa - 1543683 - IVANISE GAUBEUR
Membro Titular - Examinador(a) Externo ao Programa - 1544951 - ANA PAULA DE MATTOS AREAS DAU
Membro Titular - Examinador(a) Externo à Instituição - SÉRGIO DINIZ GARCIA
Membro Titular - Examinador(a) Externo à Instituição - GUILHERME MIOLA TITATO
Membro Suplente - Examinador(a) Interno ao Programa - 1544379 - ANDERSON ORZARI RIBEIRO
Membro Suplente - Examinador(a) Externo ao Programa - 1604147 - LEONARDO JOSE STEIL