Banca de DEFESA: ALESSANDRO LAMARCA URZEDO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ALESSANDRO LAMARCA URZEDO
DATA : 17/02/2020
HORA: 14:00
LOCAL: sala 301, 3º andar, Bloco B, Campus SA da Fundação Universidade Federal do ABC, localizada na Avenida dos Estados, 5001, Santa Terezinha, Santo André, SP
TÍTULO:

Biocompatible and antibacterial alginate hydrogels containing nitric oxide and silver nanoparticles for topical applications

PÁGINAS: 60
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Físico-Química
ESPECIALIDADE: Química de Interfaces
RESUMO:

Nitric oxide (NO) is related to several biological processes, then presenting diverse applications, as antibacterial action. Silver nanoparticles (AgNPs) are also well known for its antibacterial activity. In this work, AgNPs, biogenically synthesized using green tea (Camellia sinensis) extract, were combined with S-nitrosomercaptosuccinic acid (S-nitroso-MSA), a molecule that produces NO. A natural, biodegradable and biocompatible polymer named alginate was used to produce a hydrogel to concentrate and deliver S-nitroso-MSA and/or AgNPs. The material was characterized and healthy lineage Vero cells were used to evaluate cytotoxicity and to determine a safe concentration of application. Gram-negative bacterium Escherichia coli ATCC 25922, and two gram-positive bacteria: Staphylococcus aureus ATCC 25923 and Streptococcus mutans UA159 were used to evaluate a potential antibacterial action and to investigate a possible synergism of action between NO and AgNPs. Hydrogels containing S-nitroso-MSA showed sustained NO release for 18 h at skin temperature. S-nitroso-MSA and AgNPs containing hydrogels presented antibacterial action at 2 μg/mL hydrogel with significantly higher activity compared to hydrogel containing, individually, S-nitroso-MSA or AgNPs. This comproved a synergistic effect for antibacterial action and the in vitro cytotoxicity on Vero cells showed that concentration 2 μg/mL hydrogel is safe for healthy cells. Therefore, alginate hydrogels do have potential for topical antibacterial applications with synergism of action between NO and AgNPs.

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 1844792 - AMEDEA BAROZZI SEABRA
Membro Titular - Examinador(a) Interno ao Programa - 2351974 - JULIANA DOS SANTOS DE SOUZA
Membro Titular - Examinador(a) Externo ao Programa - 1671688 - ANDRE SARTO POLO
Membro Suplente - Examinador(a) Interno ao Programa - 2046361 - BRUNO LEMOS BATISTA
Membro Suplente - Examinador(a) Interno ao Programa - 1933328 - HELOISA FRANCA MALTEZ