Banca de DEFESA: CAMILA MOSCA BARBOZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CAMILA MOSCA BARBOZA
DATA : 14/02/2020
HORA: 10:00
LOCAL: sala 305, 3º andar, Bloco B, Campus SA da Fundação Universidade Federal do ABC, localizada na Avenida dos Estados, 5001, Santa Terezinha, Santo André, SP
TÍTULO:

EVALUATION OF THE ANTIVIRAL ACTION OF BUFOTENIN IN DIFFERENT VIRUSES OF ANIMAL AND HUMAN IMPORTANCE

PÁGINAS: 90
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
RESUMO:

The importance of viruses as etiological diseases agents and the growing discovery of new viruses has driven research in numerous areas of virology. The increased resistance of microorganisms to current available drugs available and infections that remain untreated, has motivated the search for new therapeutic alternatives. Alkaloids are compounds that have a wide variety of biological activities, including antiviral. Bufotenin is an alkaloid that can be found in vegetables and amphibian secretions and studies have already shown its antiviral potential. Canine adenovirus type 2 (CAV-2) and canine coronavirus (CCoV) are considered the main pathogens associated with canine tracheobronchitis and acute gastroenteritis in puppies respectively, in some cases with high morbidity and mortality, especially due to their ability to potentiate infections by other agents. Treatment for both infections is supportive only. Rabies is a highly fatal mammalian disease that is not effectively treated after symptoms appear. On the other hand, human herpesvirus (HSV-1) affects a large part of the world population and despite the availability of treatment, cases of resistance and toxicity have been reported. The present study evaluated the cytotoxicity of bufotenin and its antiviral potential against four virus species of medical and veterinary importance. Bufotenin activity was evaluated at non-toxic concentrations for VERO and BHK-21 cells previously determined by the MTT assay. Antiviral activity was evaluated based on the reduction of infectious viral titer in the absence and presence of bufotenin against canine adenovirus 2, canine coronavirus, human herpesvirus type 1 and rabies virus, subsequently different infectious doses of each virus were evaluated in the presence and absence of bufotenin. In viral titration, bufotenin showed significant anti-rabies activity only in the sample from non-hematophagous bat (Eptesicus furinalis). In the constant doses, the strains of domestic dog, wild canid (Cerdocyon thous) and hematophagous bat (Desmodus rotundus) and the HSV-1 KOS strain showed significant reduction. No antiviral action was observed against CAV-2, CCoV and HSV-1 resistant 29R-acyclovir strain. The results show that it was possible to identify bufotenin antiviral action against two viruses of medical importance.

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 927.634.300-82 - HELENA BEATRIZ DE CARVALHO RUTHNER BATISTA - UFRGS
Membro Titular - Examinador(a) Interno ao Programa - 2605490 - SERGIO DAISHI SASAKI
Membro Titular - Examinador(a) Externo à Instituição - KARIN CORRÊA SCHEFFER FERREIRA
Membro Suplente - Examinador(a) Interno ao Programa - 2605420 - MARIA CRISTINA CARLAN DA SILVA
Membro Suplente - Examinador(a) Externo à Instituição - WILLIAN DE OLIVEIRA FAHL