Banca de QUALIFICAÇÃO: JAINE GONCALVES GARCIA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : JAINE GONCALVES GARCIA
DATA : 18/11/2019
HORA: 14:00
LOCAL: sala 306, 3º andar, Bloco B, Campus SA da Fundação Universidade Federal do ABC, localizada na Avenida dos Estados, 5001, Santa Terezinha, Santo André, SP
TÍTULO:

In vitro characterization of different genetic lineages of rabies viruses isolated from non-hematophagous bats

 

PÁGINAS: 25
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
RESUMO:

Rabies is one of the oldest known diseases caused by rabies virus (RABV), this zoonosis has worldwide distribution and still causes the death of approximately 60,000 people per year. Rabies virus is an RNA genome virus belonging to the Rhabdoviridae family, genus Lyssavirus, and despite advances in research on this virus, there are still gaps about its maintenance in nature. Aspects related to RABV biology and its host adaptation mechanisms are important gaps that need to be filled. In recent years, the detection of RABV in different species of bats has been highlighted in Public Health. The possibility of these animals developing synanthropic habits, considering that they are reservoirs for RABV, may cause a higher risk of human infections. This paper aimed to elucidate aspects about the adaptation and maintenance of RABV in different species of non-hematophagous bats. Nine RABV samples isolated from different non-hematophagous bat species were selected. Initially the RABV samples were subjected to genetic characterization through RNA extraction, reverse transcription followed by polymerase chain reaction (RT-PCR) targeting nucleoprotein, genetic sequencing and phylogenetic analysis. Bat species were genetically identified by DNA extraction, PCR targeting mitochondrial cytochrome oxidase I (COI) and subsequent sequencing and phylogenetic analysis. After selection and characterization, the samples were submitted to intracerebral inoculation in Swiss albino mice and subsequently adapted to in vitro growth in human embryonic kidney (HEK 293T) cells. Each serial passage in the cells was subjected to the Direct fluorescent antibody test (DFAT). The following RABV genetic lineages were identified: Artibeus lituratus frugivorous bat genetic lineage (3 samples), Eptesicus furinalis insectivorous bat genetic lineage (3 samples) and Tadarida brasiliensis insectivorous bat genetic lineage (3 samples). With the genetic identification of the species, it was possible to identify that each RABV genetic lineage was kept by the respective bat species. Ten successive passages of the described samples in HEK 293T cells have been carried out so far, one of the RABV samples from the Artibeus lituratus frugivorous bat strain (IP1233/16) and one from the Eptesicus furinalis (IP964/06) are adapted to in vitro growth. With the results obtained so far, considering the analyzed samples, we can conclude that there were no cases of interspecies spillover for RABV transmission. At the end of the in vitro adaptation of the samples, they will be submitted to viral replication kinetics assays. With this work, it will be possible to propose diagnostic and surveillance tools to control specific rabies in non-hematophagous bats.

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 927.634.300-82 - HELENA BEATRIZ DE CARVALHO RUTHNER BATISTA - UFRGS
Membro Titular - Examinador(a) Interno ao Programa - 2605490 - SERGIO DAISHI SASAKI
Membro Titular - Examinador(a) Externo à Instituição - KARIN CORRÊA SCHEFFER FERREIRA
Membro Suplente - Examinador(a) Externo à Instituição - WILLIAN DE OLIVEIRA FAHL