Banca de QUALIFICAÇÃO: GIOVANA WANER CARNEIRO DE ALMEIDA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : GIOVANA WANER CARNEIRO DE ALMEIDA
DATE: 08/03/2024
TIME: 14:00
LOCAL: Sala 205 do Bloco Zeta do Campus São Bernardo da Universidade Federal do ABC
TITLE:

Expression of Human Cytomegalovirus (HCMV) IL-10 transcripts in kidney transplant recipient cells: analysis and comparison

PAGES: 75
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUBÁREA: Biologia e Fisiologia dos Microorganismos
SPECIALTY: Virologia
SUMMARY:

Human cytomegalovirus (HCMV) is an infectious agent high prevavelent worldwide that rarely causes symptomatic disease in immunocompetent individuals. However, in immunocompromised individuals, especially in transplant recipients, the virus can cause serious and even life-threatening illnesses. After primary infection, the virus remains in a latent state for life and can reactivate under certain stimuli, mainly immunosuppression. The balance between viral replication and control of the virus by the immune system is the result of a variety of viral mechanisms to evade the immune system, including viral proteins and microRNAs. One of the strategies acquired by the virus throughout evolution was the incorporation of the immunosuppressive cellular interleukin gene IL-10 (cIL-10) into its genome. The viral gene UL111A, homologous to the cIL-10 gene, produces different transcripts and isoforms of viral IL-10 through alternative splicing. The most studied are cmvIL-10 (A) and LAcmvIL-10 (B). Other isoforms have been identified, but they have been little studied, namely C, D, E, F, G and H. In previous works we showed, in vitro, that transcripts A, B, E and F are the most expressed in infected cells by the virus. In this work we sought to determine the expression of transcripts (A, B and E) in PBMCs isolated from the blood of 17 kidney transplant recipients at different times. It was identified that transcript A is the most expressed regardless of the collection date or patient. However, the expression of this transcript decreases after administration of ganciclovir treatment. The B transcript is expressed sporadically and in few samples, generally when low levels of viral DNA are detected. The E transcript was also detected in all samples, however at low expression levels that increase during the end of ganciclovir treatment. The results indicate that the cmvIL-10 (A) isoform is significantly produced in lytic and latent clinical infection. Transcript B (when identified) and E are expressed at low levels, which mostly increase when viral DNA levels are low, indicating that these transcripts are associated with HCMV latency. However, as the E transcript is identified basally in all collections, increasing its amplification when the Viral DNA drops, and the B transcript samples detected were during this drop in viral DNA amplification, we can conclude that the B transcript is more strongly associated with latency than the E.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 2605420 - MARIA CRISTINA CARLAN DA SILVA
Membro Titular - Examinador(a) Interno ao Programa - 2605490 - SERGIO DAISHI SASAKI
Membro Titular - Examinador(a) Externo à Instituição - ENRIQUE MARIO BOCCARDO PIERULIVO - USP
Membro Suplente - Examinador(a) Interno ao Programa - 1672728 - ANA CAROLINA SANTOS DE SOUZA GALVAO