Banca de QUALIFICAÇÃO: VITÓRIA LUÍZA SANTOS DAMASCENO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : VITÓRIA LUÍZA SANTOS DAMASCENO
DATE: 14/12/2023
TIME: 14:00
LOCAL: Sala 212 do Bloco Zeta do Campus São Bernardo da Universidade Federal do ABC
TITLE:

 

Investigation of the RBD domain of the gene that encodes the SARS-CoV-2 Spike protein during the UFABC community monitoring period

PAGES: 80
BIG AREA: Ciências Biológicas
AREA: Genética
SUBÁREA: Genética Molecular e de Microorganismos
SUMMARY:

 

The SARS-CoV-2 is a virus in the Coronaviridae family that emerged in Wuhan, China, in 2019 and is responsible for the global COVID-19 pandemic. This virus presents a range of clinical manifestations, from asymptomatic infections to severe cases of SARS (severe acute respiratory syndrome), affecting thousands of people and resulting in large numbers of deaths. The SARS-CoV-2 genome is composed of positive-direction RNA, protected by the nucleocapsid (N) protein. The other structural proteins of the virus are the envelope € and membrane (M) proteins, in addition to the Spike protein (S), which is responsible for the coronal shape of the virus, playing a crucial role in infection, using the receptor angiotensin-converting enzyme 2 (ACE2) to enter human cells. The S protein is the predominant molecule in the host immune response and the main component of available vaccines against COVID-19, and the evolution of RNA viruses is rapid and may have regional specificities. The ACE receptor binding domain of S protein (RBD) is the main amino acid sequence involved in the process of binding to the receptor, and its modification could reflect the ability of the virus to escape the immune system response to infection and to a vaccine. The objective of this study is to investigate the genetic variability of the SARS-CoV-2 S protein RBD domain from virus positive samples obtained during UFABC community monitoring occurred from May 2021 to December 2022. A total of 51,000 saliva samples were self-collected on cotton by members of the UFABC community, of which 1,729 were positive for SARS-CoV-2. A fragment of 430 bp corresponding to the human hypervariable mitochondrial region 1 (HV1) encoding gene was amplified by PCR from all samples, which were classified as competent by the Spike gene RT-PCR and nested-PCR which was amplified in 755 samples. The primers to amplify the S protein RBD domain were synthesized and will be used to amplify the fragment of 800 bp by RT-PCR from all 755 S positive samples, that will be sequenced, and its genetic variability characterized. This study will be the base for genetic variability detection of SARS-CoV-2 S protein RBD domain in large-scale investigations of sentinel populations.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 1675714 - MARCIA APARECIDA SPERANCA
Membro Titular - Examinador(a) Externo à Instituição - ODANIR GARCIA GUERRA - UFMS
Membro Titular - Examinador(a) Externo à Instituição - ALINE DINIZ CABRAL - SCMSP
Membro Suplente - Examinador(a) Externo à Instituição - FELIPE BAENA GARCIA