Banca de DEFESA: LUCA LOPES CESTARI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LUCA LOPES CESTARI
DATE: 07/12/2023
TIME: 14:00
LOCAL: Campus Santo André
TITLE:

ALDOSE REDUCTASE ENZYME OVEREXPRESSION IN HEK-293 CELLS: POSSIBLE MODEL OF DIABETIC NEPHROPATHY STUDIES?

PAGES: 50
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUBÁREA: Fisiologia Geral
SUMMARY:

Diabetic nephropathy is a condition responsible for a worse prognosis for diabetic patients. Its origins are attributed to several cellular pathways, but the polyol pathway and the production of sorbitol and fructose are central pieces. Even though there is a lot of research on cellular impairment pathways, studying the diabetic kidney condition remains a difficult task due to the shortage of experimental models. This study presents a methodology that seeks to conditionally express the gene limiting the polyol pathway. Microscopy analysis confirm the tool's functionality. However, tests to detect changes in genes related to osmotic stress and morphological changes did not detect expected changes. The results obtained are not sufficient to confirm that only the overexpression of the enzyme responsible for sorbitol production causes deleterious effects in an in vitro model and new methodologies can be used to study the lineage developed in other analytes.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 1653932 - MARCELO AUGUSTO CHRISTOFFOLETE
Membro Titular - Examinador(a) Interno ao Programa - 1674592 - TIAGO RODRIGUES
Membro Titular - Examinador(a) Externo ao Programa - 2353139 - ELOAH RABELLO SUAREZ
Membro Suplente - Examinador(a) Interno ao Programa - 1762353 - MARIA CAMILA ALMEIDA
Membro Suplente - Examinador(a) Interno ao Programa - 1696841 - LUCIANO PUZER
Membro Suplente - Examinador(a) Externo ao Programa - 1600878 - ARNALDO RODRIGUES DOS SANTOS JUNIOR