Banca de DEFESA: ILMA REGINA DOS SANTOS ALVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ILMA REGINA DOS SANTOS ALVES
DATE: 15/12/2023
TIME: 14:00
LOCAL: on line
TITLE:

Behavioral evaluation in rats submitted to neurodegeneration and antioxidant therapy

PAGES: 59
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Metabolismo e Bioenergética
SUMMARY:

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The study of neurodegenerative diseases has grown exponentially, influenced by the increasing number of cases of these diseases, given the increase in population life expectancy. Alzheimer's disease (AD) is one of the main causes of dementia in the elderly, and is described by the progressive decline in cognitive function and the presence of beta-amyloid protein (Aβ) plaques and neurofibrillary tangles of TAU protein. Most cases of AD, around 95% of cases, are a sporadic form that affects individuals over 65 years of age, and the main risk factor is aging. Hydroxytyrosol (HT), a polyphenolic compound found in olive oil and which is part of the Mediterranean diet, is associated with a reduced incidence of neurodegenerative diseases. Many epidemiological studies have already shown the benefits of the Mediterranean diet on the incidence of neurodegenerative diseases. This study evaluated the neuroprotective effects of HT in a model of Alzheimer's disease, evaluating behavioral measures in male Wistar rats induced to neurodegeneration by Streptozotocin (STZ) with intracerebroventricular injection (icv-STZ). Control animals received 4 μL of vehicle, pH 4.5 in citrate solution. Animals treated with STZ received 4 μL of 3 mg/kg STZ solution. Animals treated with HT + STZ received 1 μL of 450 μM HT solution and, after one hour, 3 μL of 3 mg/kg STZ. After 30 days, the animals were subjected to behavioral tests in the Barnes Maze, with 4 days of training and 1 day of final testing. During training, escape time in the escape box, distance traveled to the escape box and average speed in the maze were evaluated. In the distance covered on day 1, training 2, the group treated with STZ covered a greater distance compared to the control. The STZ + HT group showed similar results to the control group. In the final test, the only parameter that obtained a significant difference was the percentage of target exploration. Exploration in the STZ-treated group was not significantly lower than the control group. When evaluating the data comparing training on days 1 and 4, in the distance parameter there was a learning curve in the control group and in the HT+ STZ group, and not in the STZ group. Based on the results, the STZ + HT group showed more similarities with the control group than the STZ group, making it an essential tool in AD research.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 1544365 - GISELLE CERCHIARO
Membro Titular - Examinador(a) Interno ao Programa - 1672728 - ANA CAROLINA SANTOS DE SOUZA GALVAO
Membro Titular - Examinador(a) Externo ao Programa - 1994696 - SILVIA HONDA TAKADA
Membro Suplente - Examinador(a) Interno ao Programa - 1623577 - JOAO HENRIQUE GHILARDI LAGO
Membro Suplente - Examinador(a) Externo ao Programa - 3323134 - LUIZ DUARTE RAMOS