Banca de DEFESA: MICHELI COCCHI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MICHELI COCCHI
DATE: 07/12/2023
TIME: 10:00
LOCAL: por participação remota
TITLE:

PHYLOGEOGRAPHY OF RABIES VIRUS ISOLATED FROM CATTLE TRANSMITTED BY DESMODUS ROTUNDUS BLOODY BATS IN THE STATE OF SÃO PAULO

PAGES: 71
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUBÁREA: Microbiologia Aplicada
SUMMARY:

Rabies is an anthropozoonosis transmitted mainly through the bite of infected animals. This disease represents a serious public health problem, in addition to producing great economic losses to livestock. In Brazil, rabies causes the loss of approximately 45,000 cattle per year, which leads to an economic loss of up to 15 million dollars for the country's livestock. In addition to the direct loss of the animal affected by rabies, the damage to producers due to the spoliation of vampire bats and loss of leather must also be considered. In the State of São Paulo, as in the other Brazilian states, the costs of diagnostic tests, training and maintenance of professionals for the prevention of the disease must still be accounted for. Phylogenetic analysis aims to identify RABV genetic lineages and substrains, while phylogeography makes it possible to relate these genetic lineages according to their distribution over time and space. In this way, it is possible to track and understand the spatial and temporal dynamics of the disease. The objective of this work is to carry out a phylogeographic analysis of RABV in cattle, transmitted by vampire bats D. rotundus in the State of São Paulo. 73 samples of RABV isolated from cattle were selected from 2018 to 2020. These samples were subjected to genetic characterization, through RNA extraction, reverse transcription followed by polymerase chain reaction (RT-PCR) targeting the nucleoprotein, genetic sequencing and phylogenetic analysis. In the phylogenetic analysis of the 73 nucleoprotein sequences, the formation of two clades was observed, the first clade being divided into five subclades, observing 52 synonymous mutations in all groups and 11 non-synonymous mutations in important regions of the protein. In the phylogenetic analysis based on 1167 sequences, two major clades were observed, the first clade related to vampire bats, mainly from Brazil, Peru and Colombia, and the second clade related to non-hematophagous bats, mainly from the USA, Canada, Mexico and Brazil. Regarding the hosts, in clade 1, 56.99% (522/916) were bovines, 27.18% (249/916) were hematophagous bats, 7.97% (73/916) were non-hematophagous bats, 5.24% (48/916) other hosts and 2.62% (24/916) humans. The most recent ancestors of subclades 1A, 1B, 1C and 1D circulated, respectively, in 2003, 2002, 2010 and 2006. Thus, the results suggest that different lineages of RABV co-circulate, with phylogenetics being important for understanding the origin and viral distribution, as vampire bats are important reservoirs of rabies in urban and rural areas. The analysis also helps control measures of epidemiological surveillance.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 927.634.300-82 - HELENA BEATRIZ DE CARVALHO RUTHNER BATISTA VICENTE - UFRGS
Membro Titular - Examinador(a) Interno ao Programa - 2605490 - SERGIO DAISHI SASAKI
Membro Titular - Examinador(a) Externo à Instituição - JULIANA GALERA CASTILHO KAWAI - IP
Membro Titular - Examinador(a) Externo à Instituição - WILLIAN DE OLIVEIRA FAHL
Membro Suplente - Examinador(a) Externo à Instituição - PEDRO CARNIELI JUNIOR