Banca de QUALIFICAÇÃO: MARIA CATHARINA MENDES FENUCHI

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA CATHARINA MENDES FENUCHI
DATE: 28/04/2023
TIME: 14:00
LOCAL: Sala 406 do Bloco B do Campus de Santo André da Universidade Federal do ABC
TITLE:

STUDY OF IGF-1 OVEREXPRESSION ON TROPHISM AND METABOLIC RELATED GENE EXPRESSION IN TYPE II SKELETAL MUSCLE CELLS (L6)

PAGES: 70
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Hypertrophy is an adaptive process that leads to an increase in the cross-sectional area of the muscle fiber. Several factors are involved in this process: growth factors, hormones, immune system, satellite cell, among others. IGF-1 is an important regulator of muscle mass through interaction with a specific receptor (IGF-1R) that stimulates protein synthesis and inhibits protein degradation, through intracellular signaling pathways (Akt/mTOR; calcineurin/ NFAT; MAPKs and myostatin control). Peripheral insulin resistance is known to precede the development of type 2 diabetes mellitus (DMT2), and one of the causes of its development is the decrease in muscle tissue, reducing the translocation of glucose transporter 4 (GLUT4), and the increase in adipose tissue, increasing the levels of inflammatory cytokines and the accumulation of fat in myocytes, this imbalance being already established in the literature as sarcopenic obesity. Thus, the present work aims to study the overexpression of IGF-1 through the construction of a lentiviral tool controlled by the TET-On system and to evaluate the metabolic genes in L6 muscle cells, fibers of glycolytic metabolism type II, derived from muscle mouse. L6 cells were submitted to the transfection process to establish the lineage expressing the lentiviral tool (L6-TG). The L6-TG strain was submitted to doxycycline treatment to activate the TET-On system and overexpress IGF-1, in DMEM + 1% FBS Tet Free culture medium, to allow better control of gene expression. Samples obtained from this assay were evaluated qualitatively by morphological analysis and quantitatively by RT-qPCR. As a result, the activation of the TET-On system promoted overexpression of IGF-1, demonstrating efficiency in the construction of the tool, and suggesting hypertrophy in L6-TG cells. However, the data obtained by RT-qPCR did not yet demonstrate this hypertrophic process. In conclusion, from a qualitative point of view, the overexpression of IGF-1 in L6-TG cells seems to promote hypertrophy, but it was not yet possible to demonstrate the same results quantitatively, so other assessment methods are needed. 

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 1227329 - CESAR AUGUSTO JOAO RIBEIRO
Membro Titular - Examinador(a) Interno ao Programa - 1672728 - ANA CAROLINA SANTOS DE SOUZA GALVAO
Membro Suplente - Examinador(a) Interno ao Programa - 1675708 - DANIELE RIBEIRO DE ARAUJO
Membro Suplente - Examinador(a) Interno ao Programa - 1653932 - MARCELO AUGUSTO CHRISTOFFOLETE
Membro Suplente - Examinador(a) Externo ao Programa - 1600878 - ARNALDO RODRIGUES DOS SANTOS JUNIOR
Membro Suplente - Examinador(a) Externo à Instituição - GISELE GIANNOCCO - UNIFESP