Banca de QUALIFICAÇÃO: JEFERSON STABILE

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JEFERSON STABILE
DATE: 14/04/2023
TIME: 14:00
LOCAL: https://conferenciaweb.rnp.br/webconf/cristina-5
TITLE:

VASCULAR ALTERATIONS INDUCED BY THE UREMIC COMPOUND INDOXYL-SULFATE (IS): EMPHASIS ON PURINERGIC SIGNALING COMPONENTS

PAGES: 51
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUBÁREA: Fisiologia de Órgãos e Sistemas
SPECIALTY: Fisiologia Cardiovascular
SUMMARY:

Indoxyl Sulfate (IS), a solute resulting from the metabolism of the amino acid tryptophan, is a Uremic Toxin (UT) present in cases of renal insufficiency and kidney diseases. It has hypertrophic effects on the heart, fibrotic effects on the kidney, and hinders the regeneration and proper functioning of blood vessels through the action of reactive oxygen species (ROS). Purinergic signaling, summarized by the action of ectonucleotides, their receptors, and ectonucleotidases, acts to control vascular tone and inflammatory processes. Chronic activation of purinergic receptors present in the aorta of mice implies an acceleration of atherosclerosis and inflammation processes, which act synergistically with the deleterious effects of IS. In this work, the modulatory effects of IS on inflammatory and renal injury markers and on the main components of vascular purinergic signaling were evaluated. After treatment with IS 100 mg/kg/day at established times (7, 14, and 21 days), male adult C57Bl6 mice were euthanized, and the thoracic portion of the aorta was removed and cleaned. Total RNA from the aortas was extracted for gene expression analysis via qPCR. No significant variations were found in body weight, morphometric parameters, and serum urea at any of the evaluated times. On the other hand, IS significantly decreased serum creatinine values and the expression of acute-phase cytokines: IL-6, IL-1β, and TNF-α. The purinergic receptors P2X4 and P2Y6 also had their transcript levels decreased in the aorta of IS animals. No variations were found in the P2X7, P2Y1, P2Y2 receptors, and the NTPDase1, NTPDase2, and ecto-5’-nucleotidase ectonucleotidases. The results found indicate, at first, a direction towards an anti-inflammatory profile of IS, both by the decreased gene expression of cytokines and pro-inflammatory purinergic receptors. Additional experiments are necessary to understand the mechanisms involved in this modulation.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 1227329 - CESAR AUGUSTO JOAO RIBEIRO
Membro Titular - Examinador(a) Externo à Instituição - LUIZ EDUARDO BAGGIO SAVIO - UFRJ
Membro Titular - Examinador(a) Externo à Instituição - ANDREA EMILIA MARQUES STINGHEN - UFPR
Membro Suplente - Examinador(a) Interno ao Programa - 1640114 - MARCELA SORELLI CARNEIRO RAMOS