Banca de DEFESA: IMARA CARIDAD STABLE VERNIER

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : IMARA CARIDAD STABLE VERNIER
DATE: 28/04/2023
TIME: 10:00
LOCAL: Sala 302A do Bloco B do Campus de Santo André da Universidade Federal do ABC
TITLE:

CHARACTERIZATION OF MACROPHAGES AND LYMPHOCYTES IN THE ESTABLISHMENT OF THE CARDIORENAL SYNDROME TYPE 3

PAGES: 92
BIG AREA: Ciências Biológicas
AREA: Imunologia
SUBÁREA: Imunologia Celular
SUMMARY:

Cardiorenal syndrome type 3 (SCR-3) is defined as an acute renocardial syndrome and occurs upon an acute kidney injury (AKI) leading to the development of an acute cardiac injury. Currently, exist increasing appreciation of the immune system in modulating the severity of injury in experimental models of renal I/R, with studies demonstrating the potential involvement of macrophages, neutrophils, B lymphocytes, and T lymphocytes in determining the outcome of the developed AKI. The role of immune system cells in the development of SCR3 is not well established. In this sense, this work aims to characterize the macrophage and T and B lymphocyte populations in cardiac tissue after AKI induced by renal I/R. To do so, C57BL/6 mice were submitted to renal I/R protocol by occlusion of the left renal pedicle (unilateral) for 60 min, followed by reperfusion for 3, 8, and 15 days. Digestion of renal and cardiac tissues was performed and their subsequent labeling with different antibodyfluorochrome complexes to ensure the detection of each of the I/R-induced populations of interest by flow cytometry. Morphometric parameters were evaluated as well as the gene expression of immunological markers. In the study it was observed that the left kidney had a decreased renal mass at 15 days of reperfusion, demonstrating the onset of AKI. The infiltration of TCD4+ and TCD8+ lymphocytes was increased in the injured kidney after 15 days of reperfusion. A significant decrease of M1 macrophages was observed in the kidney after 3 days of reperfusion. Regarding the cardiac tissue, B cells decreased only in the I/R 8d group. Based on the results, we concluded that TCD4+, TCD8+ and, M1 macrophages cells are modulated in a tissue-specific manner in the setting of SCR3.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 1640114 - MARCELA SORELLI CARNEIRO RAMOS
Membro Titular - Examinador(a) Externo à Instituição - MARIANE TAMI AMANO
Membro Titular - Examinador(a) Externo à Instituição - RICHARDT GAMA LANDGRAF - UNIFESP
Membro Suplente - Examinador(a) Interno ao Programa - 1672728 - ANA CAROLINA SANTOS DE SOUZA GALVAO
Membro Suplente - Examinador(a) Externo ao Programa - 1844792 - AMEDEA BAROZZI SEABRA