Banca de QUALIFICAÇÃO: FELIPE BAENA GARCIA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : FELIPE BAENA GARCIA
DATE: 15/12/2022
TIME: 09:00
LOCAL: Campus São Bernardo do Campo
TITLE:

Molecular caracterization of the Trypanosoma cruzi serine protease inhibtor 2 (ISP2).

PAGES: 80
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Biologia Molecular
SUMMARY:

Ecotin, a serine peptidase inhibitor (ISP), discovered in Escherichia coli, inhibit a wide range of trypsin-like serine peptidases, protecting microorganisms from the host's immune response. In eukaryotes, ISPs encoding genes were found only in Trypanosomatidae protozoa, including the genus Trypanosoma, which harbors Trypanosoma cruzi, the ethiological agent of Chagas' disease. T. cruzi encodes the ISP2 Trypanosomatidae orthologous, which in Leishmania species present inhibitory activity on mammalian proteases from S1A family suggesting its role in vertebrate-host-parasite interactions. In this study, the structural and biochemical characterization of the recombinant T. cruzi ISP2 (rTcISP2), produced in E. coli was purified in soluble form and analyzed by circular dichroism, fluorescence spectroscopy, native electrophoresis, dynamic light scattering, low X-ray scattering and homology modeling. The obtained data revealed that rTcISP2 was biologically active and forms homodimers in solution. Furthermore, inhibitory activity of rTcISP2 against human neutrophil elastase is the highest among ecotins/ISPs orthologous from bacteria and trypanosomatids. The role of neutrophil elastase to control T. cruzi parasites through modulation of cellular and humoral innate immune responses in vertebrate hosts, make TcISP2 a key molecular component for parasite infection efficiency, providing a useful basis for investigation of host-parasite interactions and the potential of TcISP2 for biotechnological applications.

COMMITTEE MEMBERS:
Presidente - Interno ao Programa - 1675714 - MARCIA APARECIDA SPERANCA
Membro Titular - Examinador(a) Interno ao Programa - 1623577 - JOAO HENRIQUE GHILARDI LAGO
Membro Titular - Examinador(a) Externo à Instituição - LUIZ FELIPE DOMINGUES PASSERO - UNESP
Membro Suplente - Examinador(a) Interno ao Programa - 2605490 - SERGIO DAISHI SASAKI
Membro Suplente - Examinador(a) Externo ao Programa - 1838194 - RENATA SIMOES