Banca de QUALIFICAÇÃO: GABRIELLE CRISTINA MORANTE

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : GABRIELLE CRISTINA MORANTE
DATA : 03/06/2022
HORA: 09:00
LOCAL: online
TÍTULO:

Evaluation of constitutive activation of IGF-1 pathway on trophism and metabolism of type I muscle cells (Sol8)

PÁGINAS: 37
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

Introduction: Obesity, defined as the excessive accumulation of body fat, is a global epidemic known to lead to serious consequences, such as increased risk of morbidity and reduced life expectancy. Although the development of obesity is multifactorial, they all converge on a single point: energy imbalance. Skeletal muscle is a dynamic tissue that can change its phenotypic characteristics, providing better functional adaptation to varied stimuli. Hypertrophy is an adaptive process, resulting in an increase in the cross-sectional area, which involves several factors, such as: growth factors, hormones, immune system, satellite cell, among others. Insulin-like growth factor 1 (IGF-1) is an important regulator of muscle mass through interaction with a receptor called IGF receptor type 1 (IGF-1R), inducing stimuli in protein synthesis, inhibition of protein degradation or both, through of intracellular signaling pathways (Akt/mTOR; calcineurin/NFAT; MAPKs and myostatin control). A different strategy generated a constitutively active, ligand-independent variant of IGF-1R. In this study, we built a lentiviral tool governed by the Tight inducible promoter to study the constitutive activation of the IGF-1 pathway in Sol-8 cells previously expressing the TET3G system. Objective: To study the activation of the IGF-1 pathway on trophism and metabolism in Sol8 cells, derived from mouse soleus muscle (type I fibers) through constitutive activation of the chimeric receptor ErbB2V → E /IGF-1R, under control of the Tet-On system. Method: A Tight promoter-driven lentiviral tool expressing the chimeric ErbB2 V → E /IGF-1R receptor was constructed. Result: The pLENTI Tight ErbB2/IGF-1R-IRES-eGFP target vector was generated, ready for assays with the Sol-8 cell line. Conclusion: It was possible to build a lentiviral tool to control gene expression, with a universal promoter inducible by 3G TETOn system and molecular marker GFP, for overexpression of the chimeric receptor ErbB2 V → E /IGF-1R.

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 1672728 - ANA CAROLINA SANTOS DE SOUZA GALVAO
Membro Titular - Examinador(a) Interno ao Programa - 1227329 - CESAR AUGUSTO JOAO RIBEIRO
Membro Titular - Examinador(a) Externo à Instituição - WAGNER SEIXAS DA SILVA - UFRJ
Membro Suplente - Examinador(a) Interno ao Programa - 1674592 - TIAGO RODRIGUES