Banca de DEFESA: VINICIUS MANGANARO FARNEZIO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : VINICIUS MANGANARO FARNEZIO
DATA : 24/05/2021
HORA: 14:00
LOCAL: Defesa remota, via Google Meet
TÍTULO:

Developing a genetically modified strain of Zymomonas mobilis for the production of long-chain alcohols

PÁGINAS: 55
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Biologia Molecular
RESUMO:

The Gram-negative bacterium Zymomonas mobilis has several characteristics that make it attractive for the industrial production of different bioproducts, including bioethanol. For example, Z. mobilis can be grown in both aerobic and anaerobic conditions, is capable of converting glucose to ethanol more efficiently than the yeast Saccharomyces cerevisiae and tolerates higher concentrations of this alcohol during the fermentation process. However, this bacterium can be genetically modified to produce other types of biofuels and value-added products, such as isobutanol and other long-chain alcohols (LCAs), which have a higher energy density than ethanol. The current project aims to employ a genetic manipulation approach, originally developed in Escherichia coli, to construct a modified Z. mobilis strain that can be further utilized for the production of long chain alcohols. For that, two exogenous genes were cloned into Z. mobilis: a broad-spectrum 2-keto acid decarboxylase (kivd gene, from Lactococcus lactis), capable of converting 2-keto acids into aldehydes and a broad-spectrum alcohol dehydrogenase (adh2 gene from S. cerevisiae), which reduces these aldehydes to their respective alcohols. This new Z. mobilis strain, called ZmLCA1, can hopefully be used in metabolic engineering approaches, aimed at the production of isobutanol and other LCAs. Moreover, given the high toxicity of isobutanol, efforts were made to obtain a Z. mobilis strain with increased resistance to this LCA, with the aid of a directed evolution approach, based on the use of continuous culturing, in a chemostat.

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 1948411 - LUIZ ROBERTO NUNES
Membro Titular - Examinador(a) Interno ao Programa - 1696841 - LUCIANO PUZER
Membro Titular - Examinador(a) Externo à Instituição - BENY SPIRA - USP
Membro Suplente - Examinador(a) Interno ao Programa - 1674592 - TIAGO RODRIGUES
Membro Suplente - Examinador(a) Externo à Instituição - Daniela Leite Jabes - UMC