Banca de DEFESA: NAYARA UGEDA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : NAYARA UGEDA SILVA
DATA : 10/05/2021
HORA: 13:00
LOCAL: modo remoto
TÍTULO:

TRPV4 channels involvement in Tau-phosphorilation in PC12 cells exposed to cold

PÁGINAS: 55
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

Alzheimer's disease (AD) is a neurodegenerative disease and the temperature is described as a risk factor for the development of the disease. There are two histopathological markers of the disease, senile plaques of beta amyloid protein and neurofibrillary tangles (NFTs) of hyperphosphorilated tau protein. Studies indicate that cold temperatures induces an increase in P-tau, favoring the formation of NFTs, but the mechanisms involved in the transduction of the thermal signal to cells are not yet known. Thus, the thermosensitive channels of the TRP family may be the key for this mechanism. TRPV4 channels are widely expressed in the central nervous system with a thermal activation range between 27 and 34ºC, which is also conducive to the cold-induced increase in P-tau, and can be activated in pathological conditions. Therefore, this study was designed to investigate the involvement of TRPV4 channels in the hyperphosphorylation process of tau protein induced by cold in temperature in PC-12 cell culture. For that, cells were submitted to 33 and 37ºC and treated with the TRPV4 agonist, RN1747, and the TRPV4 antagonist HC067047, and their viability and P-tau levels were evaluated. The treatment with RN1747 increased the influx of calcium into the cell, and the adition of HC067047 blocked this effect. Our results demonstrated that under the temperature conditions studied, as well as blocking or activating TRPV4 pharmacologicaly  did not affect cell viability, or lead to any significant differences in the protein expression levels of TRPV4 and P-tau, suggesting a cold resistance of the cellular model.

MEMBROS DA BANCA:
Presidente - Interno ao Programa - 1227329 - CESAR AUGUSTO JOAO RIBEIRO
Membro Titular - Examinador(a) Externo ao Programa - 1771857 - CARLOS ALBERTO DA SILVA
Membro Titular - Examinador(a) Externo à Instituição - RENATA TORRES DA COSTA - MEDICINA ABC
Membro Suplente - Examinador(a) Interno ao Programa - 1672728 - ANA CAROLINA SANTOS DE SOUZA GALVAO
Membro Suplente - Examinador(a) Externo à Instituição - CINTIA MEIRELLES DE CAMARGO KOSUGI