Impact of seed irradiation on Setaria viridis: a model for the study of grasses of agronomic interest
Ionizing radiation is an abiotic factor that can exert both positive and negative effects on plants. While low doses may stimulate growth, high doses can be lethal. Understanding plant responses to different stresses enables the strategic application of stress agents to characterize adaptation and improve growth in cultivars. For instance, in environments with little or no water availability, the application of low doses of gamma radiation may stimulate the plasticity of plant adaptive mechanisms, helping plants overcome water stress. Setaria viridis, a model species of the Poaceae family, is promising for stress-related studies due to its resilience in hot and arid climates and its well-documented genetic responses to stress. The objective of this study was to investigate the effects of gamma radiation on S. viridis, aiming to evaluate whether low doses can positively modulate plant development, thereby contributing to the understanding of the potential use of this approach as a treatment strategy in agriculture under dry environmental conditions. Seeds were exposed to different doses of gamma radiation (0, 10, 100, and 1000 Gy) and, after germination in plates, were transplanted to a greenhouse. Measurements of plant height and number of leaves were taken weekly to monitor development. After 90 days, when the plants had reached their maximum growth and had dried, harvesting was carried out. At this stage, the number of inflorescences per plant, seeds per plant, plant height, and number of tillers were measured. The 10 Gy dose promoted only subtle changes compared to the control. Plants treated with 100 Gy showed slight negative variation in development. In contrast, treatment with 1000 Gy resulted in superior performance in the main growth and productivity indicators, standing out compared to the other doses. These findings reinforce the potential of S. viridis as a model species for studies on plant adaptation to unfavorable environments. The positive response to the 1000 Gy dose suggests that the controlled application of ionizing radiation may be a promising tool for inducing tolerance to abiotic stresses, with potential applications in the cultivation of other species within the Poaceae family.